A total of 290 people were arrested on Monday and Tuesday, according to London's Metropolitan police.
Environmental activists have climbed on top of a train at Canary Wharf station on the DLR in London, in a further act of civil disobedience.
Almost 300 people were arrested in London amidst ongoing climate change protests, police said late on Tuesday.
Transport officials said services were delayed.
The protests were organised by the campaign group Extinction Rebellion, which was established past year in Britain by academics and has become one of the world's fastest-growing environmental movements.
"Targeting public transport in this way would only damage the cause of all of us who want to tackle climate change, as well as risking Londoners' safety".
Extinction Rebellion spokesman James Fox said the group had attempted to maintain a blockade overnight at four sites in central London before the police came to impose the new restriction. Commuters and businesses are raging at the major disruption to at least 500,000 people as a boat continues to block Oxford Circus, activists dance on Waterloo Bridge and rows of tents cover key routes through the capital.
Extinction Rebellion said such direct action was important to bring about change.
He said he would work with TfL, the Met and the British Transport Police to minimise disruption.
Police have ordered protesters to confine their demonstrations to Marble Arch, leading to a spike in arrests on the blocked bridge.
The group is demanding the government declare a climate and ecological emergency, reduce greenhouse gas emissions to net zero by 2025 and create a citizen's assembly of members of the public to lead on decisions to address climate change.
The man and the woman said they were staging a "peaceful protest", and were planning to glue themselves to the train.
"Many of us are willing to sacrifice our liberty for the cause".
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