Neville launched a scathing attack on Guardiola while he was on punditry duty for Sky Sports last weekend, following the City manager's decision to name only six substitutes for their Premier League game against Burnley.
"We could take one from [the] second team but they played a game yesterday." he said.
"This guy, the pundit, he has to know my job is serious, it's not a joke, never is it a joke".
"If you're the academy or reserve team manager at Manchester City, you must think: 'I'm wasting my time, '" added Neville.
Management is not a book where every time you do the same things, it's not a book where you have to follow the rules.
'Of course, the prestigious pundit can say whatever he wants.
Cardiff manager Neil Warnock has hit back at Manchester City boss Pep Guardiola's calls for greater player protection following the FA Cup clash between the sides that left City winger Leroy Sane sidelined for six weeks.
'It was the first time in my life but all of the players were injured, believe me, ' he said.
City remain without Leroy Sane, Benjamin Mendy, Fabian Delph, Gabriel Jesus and David Silva.
Guardiola was furious after his side's 2-0 fourth-round win on 28 January when Sane suffered ankle ligament damage from a terrible Joe Bennett tackle.
"Leicester have the fundamentals they have from a long time ago that helped them to be champions in the Premier League", he explained.
Guardiola had hoped to have Riyad Mahrez among his options in the battle for honours on four fronts but City's bid to sign Leicester's star attacker on transfer deadline day last week fell short.
The Algerian was on strike after being denied a late move to the Etihad Stadium, but is in contention to face his potential suitors.
"After that we are not involved in absolutely anything".
Manchester City have written to the body responsible for professional referees following a number of bad tackles on their players, Press Association Sport has reported.
City have cited nine challenges they feel have not been sufficiently punished.
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